Potentiating effect of aqueous leaf extract of Anogeissus leiocarpus on Carica papaya aqueous leaf extract and Mangifera indica aqueous stem bark extract-A herbal product used against typhoid fever in Nigeria


V.N.Chidozie and G.I.Adoga.


Various aqueous concentrations of (200mg/ml, 100mg/ml, 50mg/ml, 25mg/ml and 12.5mg/ml) anogeissus leiocarpus leaf extract (AL), carica papaya leaf extract (CP) and mangifera indica stem bark extract (MI) were separately tested for antibacterial activities against salmonella typhi (the causative agent of typhoid fever) and six other bacteria using punch hole diffusion method. These three plants extract showed varying degrees of antibacterial activities as seen in the zones of inhibition of bacterial growth. Equal quantities of the plant extracts were  mixed together as follows: AL+ CP, AL+MI, CP+MI and AL+CP+MI to get four herbal preparations. These herbal preparations were separately reconstituted in sterile distilled water to get concentrations of 200mg/ml, 100mg/ml, 50mg/ml, 25mg/ml and 12.5mg/ml. These were again tested against salmonella typhi (s.typhi) and six other bacteria by agar gel diffusion method and the zones of inhibition recorded. It was noted that the antibacterial activities of the mixture of the three plant extracts was greater than those of the individual extract and the herbal mixture that does not contain anogeissus leiocarpus. This inferred that anogeissus leiocarpus aqueous leaf extract had a potentiating effect on the antibacterial activities of the other two plant extracts.


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