SufC may promote the survival of Salmonella enterica serovar Typhi in macrophages

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SufC may promote the survival of Salmonella enterica serovar Typhi in macrophages

by Sarah Lindsay May 27, 2015

Authors

Min Wang, Lin Qi, Yan Xiao, Min Wang, Chenhao Qin, Haifang Zhang, Yongmei Sheng, Hong Du

Abstract

The sufC gene of Escherichia coli (E. coli) is required for the biogenesis of iron–sulfur (Fe–S) cluster under oxidative stress conditions. In order to investigate the roles of sufC in Salmonella enterica serovar Typhi (S. Typhi), isogenic S. Typhi strain GIFU10007 harboring a non-polar mutation of sufCsufC) was constructed and the results showed that the sufC deleted mutant grew more slowly than the wild type strain when encounter oxidative stresses. Moreover, the deletion of sufC gene decreased S. Typhi survival within macrophages. After macrophages infected by sufC deleted mutant and wild type strain, we detected IL-6 and TNF-α released into the supernatant, and found the expression of IL-6 and TNF-α decreased in the supernatant of sufC deleted mutant infected groups than the wild type strain infected ones. In summary, our results showed that SufC may promote S. Typhi coping oxidative stress and help S. Typhi survival in macrophages.

 

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